• Login
    View Item 
    •   Mak IR Home
    • College of Health Sciences (CHS)
    • School of Bio-Medical Sciences (Bio-Medical)
    • School of Bio-Medical Sciences (Bio-Medical) Collections
    • View Item
    •   Mak IR Home
    • College of Health Sciences (CHS)
    • School of Bio-Medical Sciences (Bio-Medical)
    • School of Bio-Medical Sciences (Bio-Medical) Collections
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Prevalence and genotypic analysis of entamoeba histolytica isolates from Soroti by RFLP of serine-rich entamoeba histolytica protein gene locus

    Thumbnail
    View/Open
    ekou-covab-masters.pdf (1.541Mb)
    Date
    2012-11
    Author
    Ekou, Justine
    Metadata
    Show full item record
    Abstract
    Amoebiasis is an infection caused by water borne protozoan parasite Entamoeba histolytica. In Uganda where sanitation infrastructure and health education is not adequate, amoebiasis is still an important health problem. However there is little or no data on prevalence of this very important protozoan infection. In addition, microscopy remains the main method for the diagnosis of amoebiasis but cannot differentiate between Entamoeba dispar and Entamoeba histolytica infections. This has made determination of the prevalence of Entamoeba histolytica infections difficult. Hence there is need to perform molecular characterization of E. histolytica and E. dispar species. This study used both microscopy and polymerase chain reaction (PCR) to study prevalence and genotypic diversity of Entamoeba histolytica among humans in Soroti district, Eastern Uganda. Direct examination of wet stool, direct PCR and RFLP of the SREHP gene was performed. The overall prevalence of amoebiasis was 19.93%. Infection with Entamoeba histolytica/dispar was higher among males (22.14%) compared to females (17.39%). The 6-12 years age group recorded the highest prevalence at 24.44% but it was lowest in the 13-19 years age group at 17.65% (P<0.05). It was shown that only 36.7% (n=22) of the samples initially diagnosed as positive by microscopy were positive by PCR. The degree of agreement between microscopy and PCR was moderate (k=0.48). The prevalence of E. histolytica and E.dispar was found to be 7.31% and 12.6% respectively. Entamoeba histolytica isolates in the study area had eight different RFLP patterns. In conclusion, amoebiasis is an important public health problem in the study area and Entamoeba histolytica isolates are diverse in terms of strains.
    URI
    http://hdl.handle.net/10570/2470
    Collections
    • School of Bio-Medical Sciences (Bio-Medical) Collections

    DSpace 5.8 copyright © Makerere University 
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of Mak IRCommunities & CollectionsTitlesAuthorsBy AdvisorBy Issue DateSubjectsBy TypeThis CollectionTitlesAuthorsBy AdvisorBy Issue DateSubjectsBy Type

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DSpace 5.8 copyright © Makerere University 
    Contact Us | Send Feedback
    Theme by 
    Atmire NV