• Login
    View Item 
    •   Mak IR Home
    • College of Agricultural and Environmental Sciences (CAES)
    • School of Agricultural Sciences (SAS)
    • School of Agricultural Sciences (SAS) Collections
    • View Item
    •   Mak IR Home
    • College of Agricultural and Environmental Sciences (CAES)
    • School of Agricultural Sciences (SAS)
    • School of Agricultural Sciences (SAS) Collections
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Abundance and diversity of tomato rhizosphere microbes in different land uses and their effect on bacterial wilt disease in Uganda

    Thumbnail
    View/Open
    Masters dissertation (1.076Mb)
    Date
    2021-04-26
    Author
    Nampamya, Doreen
    Metadata
    Show full item record
    Abstract
    Agricultural production is associated with changes in microbial diversity. This often leads to microbial unevenness and multiplication of soil borne diseases such as wilts, which are tedious to control and trend managed through use of biocontrols. Microbiome composition and land use management have been mentioned as core factors in effectiveness of biocontrols. Therefore, this study determined the diversity and abundance of microbes in the tomato rhizosphere and its effect on severity and biocontrol of bacterial wilt disease in soils from different land uses . Tomatoes were grown in a screen house at Namulonge using unsterilized soils from cultivated, fallow and forest land use types and were inoculated with Ralstonia solanacearum (positive control) but also include a non-inoculated set (negative control). Four biocontrols were used namely; Bacillus subtilis, Bacillus amyloliquefaciens, Peanibacillus polymyxa and Stenotrophomonas rhizophila. Data were collected on plant growth and yield parameters, and bacterial wilt disease incidence. Rhizosphere microbiome data were obtained using amplicon sequencing. Results showed that B. subtilis and P. polymyxa increased plant height in all land use types. Biocontrols B. amyloliquefaciens and S.rhizophila resulted into the highest dry biomass in cultivated land use, B. amyloliquefaciens and P. polymyxa in fallow and P. polymyxa and B. subtilis in forest land use type. In cultivated land use, biocontrols B. amyloliquefaciens, B. subtilis and S. rhizophila reduced bacterial wilt incidence by 31 % and P. polymyxa by 28 %. Fallow land use type had the highest microbial diversity and forest land use had the highest microbial abundance. The major phyla were Proteobacteria, Actinobacteria and Acidobacteria in Forest, Fallow and cultivated land uses respectively. Bacillus and Paenibacillus bacteria were more abundant in cultivated while Stenotrophomonas bacteria were more abundant in forest land use type
    URI
    http://hdl.handle.net/10570/9391
    Collections
    • School of Agricultural Sciences (SAS) Collections

    DSpace 5.8 copyright © Makerere University 
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of Mak IRCommunities & CollectionsTitlesAuthorsBy AdvisorBy Issue DateSubjectsBy TypeThis CollectionTitlesAuthorsBy AdvisorBy Issue DateSubjectsBy Type

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DSpace 5.8 copyright © Makerere University 
    Contact Us | Send Feedback
    Theme by 
    Atmire NV