• Login
    View Item 
    •   Mak IR Home
    • College of Health Sciences (CHS)
    • School of Health Sciences (Health-Sciences)
    • School of Health Sciences (Health-Sciences) Collections
    • View Item
    •   Mak IR Home
    • College of Health Sciences (CHS)
    • School of Health Sciences (Health-Sciences)
    • School of Health Sciences (Health-Sciences) Collections
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Antimicrobial susceptibility of 3rd generation Cephalosporins-resistant escherichia coli, Klebsiella pneumoniae and Enterobacter spp isolates carried in the gut of patients on admission to Mulago Hospital

    Thumbnail
    View/Open
    Master's Dissertation (2.736Mb)
    Date
    2019-11-11
    Author
    Kiiza, Hillary
    Metadata
    Show full item record
    Abstract
    Background: Resistance to 3rd and 4th generation cephalosporin is on an increase worldwide. These cephalosporins are used in management of severe infections caused by Enterobacteriaceae. The main objective of the study was to determine the susceptibility of third generation cephalosporins-resistant Escherichia coli, Klebsiella pneumoniae and Enterobacter spp isolated from patients at Mulago Hospital. Methods: This was a cross-sectional study that looked at 80 Enterobacteriaceae Isolates, 42 Escherichia coli, 30 Klebsiella pneumoniae and 8 Enterobacter species that were isolated from rectal samples of patients on admission to Mulago Hospital. Antimicrobial susceptibility testing was done using amoxicillin/clavulanate, ampicillin/sulbactam, piperacillin/tazobactam, cefotaxime, ceftriaxone, ceftazidime, aztreonam, ertapenem, imipenem, meropenem, tigecycline, fosfomycin, trimethoprim-sulfamethoxazole, gentamicin, amikacin, nalidixic acid, nitrofurantoin, chloramphenicol, cefoxitin, ciprofloxacin, cefepime,ampicilin and tetracycline discs. The plates were incubated and interpreted according to CLSI guidelines after measuring the zone diameters. For Tigecycline the FDA guidelines were used. Agar dilution method for testing susceptibility of cefoperazone-sulbactam was used on three concentrations (16µg/ml, 32 µg/ml and 64 µg/ml) of cefoperazone/sulbactam dissolved in MHA. Phenotypic ESBL production was detected using the Double Disc Synergy Test. The modified inhibition carbapenamase test was used for detection of carbapenamase production. OXA gene variants were detected using PCR. The PCR products were then sequenced. Results: Results show that 77(96.2 %) were ESBL producers while 3 (3.8 %) were of non-ESBL phenotype. E.coli was the most isolated organism 42(52.5 %), followed by K.pneumoniae 30 (37.5 %) and Enterobacter spp 8(10%).E.coli had the highest percentage of ESBL producers 41(97.6%). Susceptibility was the highest 75(93.8%) to Meropenem and Ertapenem. There was no isolate recorded that produced AmpC and carbapenamase enzymes. 20% of the isolates were positive for OXA-1, 7.5% for OXA-10 and 6.25% for OXA-48. Conclusions: This study reveals a high resistance to drug-inhibitor combinations and cefepime among E. coli, K. pneumoniae and Enterobacter spp that are resistant to 3rd generation cephalosporins isolated from rectal samples of patients on admission to Mulago Hospital.
    URI
    http://hdl.handle.net/10570/7658
    Collections
    • School of Health Sciences (Health-Sciences) Collections

    DSpace 5.8 copyright © Makerere University 
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of Mak IRCommunities & CollectionsTitlesAuthorsBy AdvisorBy Issue DateSubjectsBy TypeThis CollectionTitlesAuthorsBy AdvisorBy Issue DateSubjectsBy Type

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DSpace 5.8 copyright © Makerere University 
    Contact Us | Send Feedback
    Theme by 
    Atmire NV