Show simple item record

dc.contributor.authorSeni, Jeremiah
dc.date.accessioned2014-06-30T11:11:17Z
dc.date.available2014-06-30T11:11:17Z
dc.date.issued2012-06
dc.identifier.citationSeni, J. (2012). Molecular epidemiology of methicillin resistant staphylococcus aureus in patients with surgical site infections at Mulago Hospital, Kampala – Uganda. Unpublished master's thesis, Makerere University, Uganda.en_US
dc.identifier.urihttp://hdl.handle.net/10570/3020
dc.descriptionA dissertation submitted to the School of Graduate Studies in partial fulfillment of the requirements for award of the degree of Master of Medicine in Microbiology of Makerere University.en_US
dc.description.abstractBackground: Surgical site infections (SSI) are globally the third most common type of nosocomial infections with Staphylococcus aureus being the most common causative agent. SSI are difficult to treat and are associated with substantial longer hospital stay, higher cost, morbidity and mortality particularly when the etiological agent is Methicillin resistant Staphylococcus aureus (MRSA). In a recent study, MRSA accounted for 31.5% of Staphylococcus aureus isolates in patients with SSI at Mulago National Hospital, however, MRSA lineages circulating in Mulago surgical patients are not known. Further, it is not known whether there is clonal spread of the strains. Therefore, this study aimed at determining MRSA lineages in Mulago surgical patients using SCCmec and spa genotyping techniques. Data herein will also be helpful in guiding infection control at Mulago National Hospital. Materials and methods: A cross sectional study was conducted from September 2011 to April 2012 and involved 314 patients with SSI in the general surgery, orthopedic surgery and obstetrics & gynaecology wards at Mulago National Hospital. Microbiological and Molecular procedures were performed in the Bacteriology and Molecular laboratories of the Department of Medical Microbiology, Makerere University College of Health Sciences. Pus swabs were taken from SSI and processed with conventional phenotypic methods to identify MRSA, which were confirmed by PCR-detection of the mecA gene. MRSA were also screened for presence of pvl gene, and further genotyped by multiplex PCR to determine SCCmec types. Then, spa sequence based genotyping was done on all S. aureus isolates to determine their genetic lineages. Results: Of the 314 patients with SSI, Staphylococcus aureus accounted for 20.4% (64/314), of which 37.5% (24/64) were MRSA. The predominant SCCmec types were type V 33.3% (8/24) and type I, 16.7% (4/24). Based on spa genotyping, the predominant lineages were t645 in 17.2% (11/64) and t4353 in 15.6% (10/64), of these MRSA accounted for 8.3% (2/24) and 4.2% (1/24) respectively. These spa lineages were found to be clonally circulating in all surgical wards. Conversely, lineages t064, t355, and t4609 were confined to the obstetrics and gynecology wards. Moreover, a new spa type namely t10277 was identified in this study. MRSA SSI was strongly associated with multidrug resistance, inducible clindamycin resistant (p-value 0.002), infections involving organ (0.005) and cancer comorbid (0.022) as compared to MSSA SSI. The finding of VRSA isolate in this study may be the first in the region. Conclusions: In this study, SCCmec types V and I are the most prevalent MRSA genotypes. The predominant spa lineages (t645 and t4353) were found to be clonally circulating in all surgical wards. Recommendations: The finding of predominant spa types circulating in all surgical wards calls for strengthening of infection control practices at Mulago National Hospitalen_US
dc.language.isoenen_US
dc.publisherMakerere Universityen_US
dc.subjectMolecular Epidemiologyen_US
dc.subjectMethicillinen_US
dc.subjectStaphylococcus Aureasen_US
dc.subjectMulago Hospital, Ugandaen_US
dc.titleMolecular epidemiology of methicillin resistant staphylococcus aureus in patients with surgical site infections at Mulago Hospital, Kampala – Ugandaen_US
dc.typeThesisen_US


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record