Biopreservation of brycinus nurse fish (Onangananga) using beeswax and thyme essential oil with complementary packaging

dc.contributor.author Mannakhe, Moreen Dianah
dc.date.accessioned 2024-12-10T08:50:17Z
dc.date.available 2024-12-10T08:50:17Z
dc.date.issued 2024-12
dc.description A dissertation submitted to the College of Agricultural and Environmental Sciences in partial fulfillment of the requirements for the award of a Master of Science Degree in Food Science and Technology of Makerere University en_US
dc.description.abstract Bio-coatings maintain food quality by minimizing exchange of oxygen, light and moisture across the food matrix, hence inhibiting microbial proliferation. Including essential oils in the bio-coatings enhances flavor, antioxidant and antimicrobial properties thereby extending shelf life. This study aimed to evaluate the preservation effectiveness of beeswax and thyme essential oil coatings on sun-dried Brycinus nurse fish (Onangnanga) obtained from Panyimur market, Pakwach district, Uganda. Sun-dried B.nurse samples were stored under ambient conditions in open (box), vacuum, and container packaging for six weeks. Analyses were conducted weekly to assess microbial quality (Salmonella, Pseudomonas, E. coli, Staphylococcus, and total plate counts (TPC)) and, biochemical properties (pH, moisture content, total volatile basic nitrogen (TVBN), and Thiobarbituric acid reactive substances (TBARS)). Sun-dried B.nurse exhibited low microbial quality with TPC of 7 log CFU/g, detectable levels of Salmonella spp, E.coli spp. ranged from 3.56 to 3.74 log CFU/g and Staphylococcus spp from 1.11 to 6.9 log CFU/g. Moisture content significantly (p < 0.05) increased from 15.06% during storage as follows: Container packaging to 28.81%, vacuum package to 16.26%, beeswax coating to 26.1% and thyme with beeswax coating to 21.80%. TVBN levels significantly increased (p< 0.05) from 24.12 mgN/100g to 35.61 mgN/100g, with the different treatments as follows: container packaging (34.39 mgN/100g), vacuum packaging (35.61 mgN/100g) beeswax coating (32.08 mgN/100g) and thyme with beeswax coating (30.83 mgN/100g). TBARS values increased significantly (p<0.05) from 4.03 mgMDA/kg to 18.25 mgMDA/kg, with container packaging (11.81 mgMDA/kg), vacuum packaging (15.14 mgMDA/kg), beeswax coating (12.46 mgMDA/kg) and thyme with beeswax coating (9.92 mgMDA/kg). The pH values remained stable regardless. Both packaging types and coatings significantly (p<0.05) influenced the quality of sun-dried B. nurse. This study showed that beeswax and thyme essential oil coatings combined with suitable packaging preserved the quality of sun-dried Brycinus nurse , hence effectively extending its shelf life by 21 days. en_US
dc.identifier.citation Manakhe, M. D. (2024). Biopreservation of brycinus nurse fish (Onangananga) using beeswax and thyme essential oil with complementary packaging; unpublished dissertation, Makerere University, Kampala en_US
dc.identifier.uri http://hdl.handle.net/10570/13939
dc.language.iso en en_US
dc.publisher Makerere University en_US
dc.subject Biopreservation en_US
dc.subject Thyme essential oil and packaging en_US
dc.subject Brycinus nurse en_US
dc.title Biopreservation of brycinus nurse fish (Onangananga) using beeswax and thyme essential oil with complementary packaging en_US
dc.type Thesis en_US
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