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Performance of two commercial immunochromatographic assays for rapid detection of antibodies specific to human immunodeficieny virus types 1 and 2 in serum and urine samples in a rural community-based research setting (Rakai, Uganda)

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dc.contributor.author Kagulire, S. C.
dc.contributor.author Stamper, P. D.
dc.contributor.author Opendi, P.
dc.contributor.author Nakavuma, J. L.
dc.contributor.author Mills, L. A.
dc.contributor.author Makumbi, F.
dc.contributor.author Gray, R. H.
dc.contributor.author Serwadda, D.
dc.contributor.author Reynolds, S. J.
dc.date.accessioned 2012-06-21T08:30:40Z
dc.date.available 2012-06-21T08:30:40Z
dc.date.issued 2007-06
dc.identifier.citation Kagulire, S.C., Stamper, P.D., Opendi, P., Nakavuma, J.L., Mills, L.A., Makumbi, F., Gray, R.H., Serwadda, D., Reynolds, S.J. (2007). Performance of two commercial immunochromatographic assays for rapid detection of antibodies specific to human immunodeficiency virus types 1 and 2 in serum and urine samples in a rural community-based research setting (Rakai, Uganda). Clinical and Vaccine Immunology, 14(6) en_US
dc.identifier.issn 1556-6811
dc.identifier.uri http://hdl.handle.net/10570/621
dc.description.abstract Rapid detection of human immunodeficiency virus (HIV) antibodies is of great importance in developing and developed countries to diagnose HIV infections quickly and at low cost. In this study, two new immunochromatographic rapid tests for the detection of HIV antibodies (Aware HIV-1/2 BSP and Aware HIV-1/2 U; Calypte Biomedical Corporation) were evaluated in rural Africa to determine the tests’ performance and comparability to commercially available conventional enzyme immunoassay (EIA) and Western blot (WB) tests. This prospective study was conducted from March 2005 through May 2005 using serum and urine from respondents in the Rakai Community Cohort Survey. Nine hundred sixty-three serum samples were tested with the Aware blood rapid assay (Aware-BSP) and compared to two independent EIAs for HIV plus confirmatory Calypte WB for any positive EIAs. The sensitivity of Aware-BSP was 98.2%, and the specificity was 99.8%. Nine hundred forty-two urine samples were run using the Aware urine assay (Aware-U) and linked to blood sample results for analysis. The sensitivity of Aware-U was 88.7% and specificity was 99.9% compared to blood EIAs confirmed by WB analysis. These results support the adoption of the Aware-BSP rapid test as an alternative to EIA and WB assays for the diagnosis of HIV in resource-limited settings. However, the low sensitivity of the Aware-U assay with its potential for falsely negative HIV results makes the urine assay less satisfactory. en_US
dc.language.iso en en_US
dc.publisher American Society for Microbiology en_US
dc.subject HIV/AIDS en_US
dc.subject Community based research en_US
dc.subject Rakai District en_US
dc.subject Uganda en_US
dc.title Performance of two commercial immunochromatographic assays for rapid detection of antibodies specific to human immunodeficieny virus types 1 and 2 in serum and urine samples in a rural community-based research setting (Rakai, Uganda) en_US
dc.type Journal article, peer reviewed en_US


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